MS培养基应加多少琼脂和蔗糖?

2025-04-06 04:42:27
推荐回答(2个)
回答1:

  蔗糖30g /L,琼脂7 g /L。
  MS培养基是Murashige和Skoog于1962年为烟草细胞培养设计的,其特点是无机盐和离子浓度较高,是较稳定的离子平衡溶液,它的硝酸盐含量高,其养分的数量和比例合适,能满足植物细胞的营养和生理需要,因而适用范围比较广,多数植物组织培养快速繁殖用它作为培养基的基本培养基。 基于此,这种培养基就用他们的名字来命名了。
  MS培养基是目前使用最普遍的培养基。其具有较高的无机盐浓度,能够保证组织生长所需的矿质营养还能加速愈伤组织的生长。由于配方中的离子浓度高,在配制、贮存和消毒等过程中,即使有些成分略有出入,也不会影响离子间的平衡。MS固体培养基可用于诱导愈伤组织,也可用于胚、茎段、茎尖及花药的培养,其液体培养基用于细胞悬浮培养时能获得明显的成功。MS培养基的无机养分的数量和比例比较合适,足以满足植物细胞在营养上和生理上的需要。因此,一般情况下,不用再添加氨基酸、酪蛋白水解物、酵母提取物及椰子汁等有机附加成分。和其它培养基的基本成分相比,MS培养基中的硝酸盐、钾和铵的含量高,这是它的显著特点。
  配方单:
  mg/L工作液浓度 单位
  大量元素:
  NH4NO3 1650
  KNO3 1900
  CaCl2·2H2O 440
  MgSO4·7H2O 370
  KH2PO4 170
  微量元素:
  KI 0.83
  H3BO3 6.2
  MnSO4·4H2O 22.3
  ZnSO4·7H2O 8.6
  Na2MoO4·2H2O 0.25
  CuSO4·5H2O 0.025
  CoCl2·6H2O 0.025
  铁盐:
  FeSO4·7H2O(27.8)
  Na2-EDTA·2H2O(37.3)
  有机物质:肌醇100
  烟酸0.5
  盐酸吡哆醇(维生素B6) 0.5
  盐酸硫胺素(维生素B1) 0.1
  甘氨酸2.0
  注意:肌醇一般是要另外分开来独自配成浓缩液,因为它比较难溶,一般配成100倍,大量元素可以配成1000倍的。
  

回答2:

5.5克琼脂30克蔗糖

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