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Egg yolk antibody is of great potential for development of antibodies resources. In the appropriate ion concentration and pH conditions, the bitterness with the majority of the yolk protein response, the formation of irreversible precipitation, the only category IgY antibody protein does not react, remain in supernatant. Experimental use of the sorrows of precipitation from egg yolk antibody, and to optimize extraction conditions. Results in the sorrows of two percent concentration, water dilution than 1:6, pH 5.5 can be achieved under the conditions better extraction, access to the yolk extracts of the HI titer to 1:512 (HI 9). That bitterness precipitation from egg yolk antibody is simple, fast and effective advantages, with the further development and utilization of the value.
Abstract
Egg yolk antibody is of great potential for development of antibodies resources. In the appropriate ion concentration and pH conditions, the bitterness with the majority of the yolk protein response, the formation of irreversible precipitation, the only category IgY antibody protein does not react, remain in supernatant. Experimental use of the sorrows of precipitation from egg yolk antibody, and to optimize extraction conditions. Results in the sorrows of two percent concentration, water dilution than 1:6, pH 5.5 can be achieved under the conditions better extraction, access to the yolk extracts of the HI titer to 1:512 (HI 9). That bitterness precipitation from egg yolk antibody is simple, fast and effective advantages, with the further development and utilization of the value.
Key words: Yolk antibodies, bitterness precipitation, hemagglutination inhibition, antibody titers
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