An immune-and micro-column - fluorescence method is immune affinity chromatography column - Fluorescence Spectrometer (IAC-SFB) base, using self-developed immune pro - and micro-Immunoaffinity replace imports and, in a aflatoxin rapid quantitative detection, and the immune affinity column - Fluorescent Light meter (IAC-SFB) that the United States VICAM company fungal toxin fluorescence analyzer test this quantitative method, AFT recoveries were 80% and above, the coefficient of variation is less than 10%, indicating that the measured results are accurate and reliable, good repeatability.
Two, this study also aflatoxin rapid quantitative detection method based on the simple combination of fluorescence analyzer and adsorption column, the development of rapid detection of aflatoxin, which pro - and micro-immune - adsorption column - fluorescence assay (IAC-SM-SFB). The performance indicators show that the blank samples for the recovery of AFT add roughly ~ ppb detection limit, below the national standards set forth in the ppb; Added experimental antibodies for the detection limit recovery for 0.2ppb ~ 0.3ppb, below the national standards set forth in the 0.5ppb have to meet the requirements of national standards. And with immune-and micro-column - fluorescence method validation of this qualitative method, with high sensitivity, fast, safe and easy operation, low cost, specificity, broad applicability, and high-throughput characteristics.
3, admitted that the samples used in this study to establish two rapid detection of aflatoxin detection of aflatoxin contamination, the two methods in line with the test results. To illustrate the two methods can be of the AFT, quantitative method for the AFT pollution confirmed qualitative method can meet the screening requirements at the scene.
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