对组培生产的几个疑问,希望能得到专业人士的回答!

2025-03-16 12:07:08
推荐回答(2个)
回答1:

1.如果资金允许当然有风淋间最好了,没有也能用,毕竟还有超静台吹风,很多大学实验室也没有单独的风淋间。但建议在外间和操作间之间建个缓冲间,换衣服和拖鞋什么的。
2.MS干粉没用过,但估计比自己配置的MS培养液贵,毕竟人家是商品化的方便利用的东西么。调PH值一般情况下需要添加的是NAOH,也就是说培养基和激素组合后一般情况下PH是偏酸的,当然这要看你培养基里添加的成分而异,一般情况下是偏酸的。
3.理论上组培过程中产生变异的可能性非常小,因此可以多次使用继代苗作为快繁的母株。但不可否认长期使用继代苗作为繁殖材料有可能会产生变异,也就是生长出的东西可能会发生表形上的变化,因此如果是商品化生产苗木最好要隔一定的继代次数后重新选取优良材料进行你讲的外植体诱导不定芽,具体多少代为宜不同的植物有一定的差别,可能需做一定研究才能下结论,但个人认为10代以内应该问题不大吧。继代操作就是把增值出的芽分装到新培养基里进行继续增殖或者保存母本。丛生芽可以从芽丛底部分成小的芽丛团进行继代增殖,当然需要你进行适当修剪,比如去掉老叶和粗枝及可能会产生的根等。
敲了这么多字辛苦啊,给分吧。

回答2:

地方

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