推荐回答（5个）
不可以做主语,但可以位于句首.
可做主语的词类包括：名词,代词,动名词或不定式短语,名词性从句,名词短语,表距离的地方副词
下面那位同学举的这个例子是口语化的不符合语法规律的。
书面语中，介词短语做主语其实是【倒装句】的一种体现形式。
例如：On top of the shelf is the champion cup that he is most proud of.
on top of the shelf看似是主语，the champion cup是宾语，但其实正常的语序是
The champion cup that he is most proud of is on top of the shelf.
the champion cup是主语，on top of the shelf是表语。
词是不可以做主语的，介词短语一般做状语使用，主语一般是一些名词。不可以做主语的，代词，动名词，动词的不定式形式等等
介词不可以做主语，介词短语一般做状语使用。不可以做主语的，主语一般是一些名词，代词，动名词，动词的不定式形式等等，但是介词短语是可以放在句首的，引起倒装 的哦。
选我选我选我~~~~~
之前回答的答案纯属扯淡，谁说介词短语不能做短语？举个例子, between 5 o.clock and 6 o.clock is good for me.这就是介词短语最主语啊，一帮人不会在那里扯淡。
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