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中英文翻译高手高手高高手请进（修改翻译）

2025-02-01 13:23:27

推荐回答（3个）

回答1：

由于SMART技术是利用逆转录酶内源的末端转移酶活性，只要单管，一步即可完成，不需要额外的cDNA抽提纯化或者沉淀，或者额外的酶反应，只需要少至25ng的mRNA或者50ng的Total RNA就可以得到高质量、高产量的cDNA库，更重要的是得到的cDNA能够代表原有样品中的mRNA的丰度，因此在本实验中把它直接用于构建绒山羊（Capra hircus）毛囊兴盛期皮肤组织的cDNA质粒文库。Trizol Reagent（GIBCO/BRL）分离RNA后，需要提取mRNA，这需要用Oligotex（QIAGEN），提取步骤如下：以锚定引物反转录合成cDNA第一链作为模板链，以两个锚定引物，用长链PCR扩增全长cDNA双链。用SfiⅠ酶切去除小片段后的CHROMA SPIN-400，连接到SfiⅠ消化的pBluescriptⅡ SK（带有SfiⅠA和B两个位点）质粒载体中，转化E.coli 5a，库容量为1.8×105clones。通过对文库中克隆子的序列测定和生物信息学初步分析，并且用B lastX及B lastN软件进行同源对比，得到1200多条EST序列，本篇文章就从中选取20多条进行分析，辽宁新品系绒山羊cDNA文库以及EST文库的成功构建,为研究辽宁新品系绒山羊功能基因和蛋白质组学奠定了基础。

New Strain of Liaoning cashmere goat skin, hair follicles hugely popular for a cDNA library construction and EST sequence analysis
Abstract: The SMART technology is the use of reverse transcriptase endogenous terminal transferase activity, as long as the single-tube, one step can be completed, no additional purification or precipitation of the cDNA extraction, or an additional enzymatic reactions, just as little as 25ng of the Total RNA or 50ng of mRNA can get high-quality, high-yield cDNA library, more importantly, to be able to represent the original sample of cDNA of mRNA abundance, so in this experiment used to construct it directly to cashmere goat (Capra hircus) follicle hugely popular skin tissue cDNA plasmid library. Trizol Reagent (GIBCO / BRL) separation of RNA, you need to extract the mRNA, which requires using Oligotex (QIAGEN), extraction steps are as follows: the anchor primer first-strand cDNA synthesis of reverse transcription as a template chain, with two anchor primers, using PCR amplification of long-chain full-length double-stranded cDNA. Digested with Sfi Ⅰ after the removal of small fragments CHROMA SPIN-400, connect to the Sfi Ⅰ digested pBluescript Ⅱ SK (with Sfi Ⅰ A and B 2 sites) plasmid vector and transformed into E.coli 5a, storage capacity of 1.8 × 105clones . Library clones by sequence determination and bio-informatics preliminary analysis, and with the B lastX and B lastN software homologous comparison, received more than 1,200 of EST sequences, this article will select from more than 20 analysis, Liaoning New strain cashmere goats EST library cDNA library as well as the successful construction of new lines for the study of Liaoning cashmere goats functional genomics and proteomics basis.

回答2：

Prosperous scheduled time of new strain fine hair mountain wool bag skin cDNA library structure builds Liaoning reaching EST array analysis Abstract: Since the SMART technology is to make use of the end reversing the record enzyme inner source to change an enzymatic activity , not to require that extra cDNA is extract the purification or precipitayion , possibly extra as long as the Shan tube , single-step are OK to be completed, enzyme reaction, only need few extremely 25 ng mRNA or 50 ng Total RNA right away not bad high of high quality, output cDNA warehouse, more important being cDNA can representative original mRNA in sample abundance, Use it to structure the cDNA quality granule library that prosperous scheduled time of fine hair goat (Capra hircus) hair follicle skin organizes therefore directly in the experiment. Trizol Reagent (GIBCO/BRL) separates the RNA queen , needs to extract mRNA, this needs to use Oligotex (QIAGEN), to extract a step as follows: Cause thing reverse transcription to compose cDNA being form board chain the first cable length with anchorage, anchorage attracts a thing with two, use long-chain PCR to expand increasing the overall length cDNA double strand. Use Sfi I enzyme to be sure to dislodge CHROMA of the young fragment queen SPIN-400, 5 as linking up to changing E.coli in the pBluescript II SK (two unit burns with Sfi I A and B) quality granule carrier that Sfi I digests,warehouse capacities are 1.8 X 105 clones. By the fact that information learns the first step analysis to the array admeasurement and living things overcoming Long Zi county in the library, use B lastX and B lastN software to carry out the consanguinity contrast and , get more than 1,200 EST array, the article chooses the analysis more than 20 being in progress originally right away out of , new Liaoning strain fine hair goat cDNA library and EST library success build build, the function gene and proteide study of group have establish a basis for the strain fine hair goat studying Liaoning is new.

回答3：

Abstract: The SMART technology is the use of reverse transcriptase endogenous terminal transferase activity, as long as the single-tube, one step can be completed, no additional purification or precipitation of the cDNA extraction, or an additional enzymatic reactions, just as little as 25ng of the Total RNA or 50ng of mRNA can get high-quality, high-yield cDNA library, more importantly, to be able to represent the original sample of cDNA of mRNA abundance, so in this experiment used to construct it directly to cashmere goat (Capra hircus) follicle hugely popular skin tissue cDNA plasmid library. Trizol Reagent (GIBCO / BRL) separation of RNA, you need to extract the mRNA, which requires using Oligotex (QIAGEN), extraction steps are as follows: the anchor primer first-strand cDNA synthesis of reverse transcription as a template chain, with two anchor primers, using PCR amplification of long-chain full-length double-stranded cDNA. Digested with Sfi Ⅰ after the removal of small fragments CHROMA SPIN-400, connect to the Sfi Ⅰ digested pBluescript Ⅱ SK (with Sfi Ⅰ A and B 2 sites) plasmid vector and transformed into E.coli 5a, storage capacity of 1.8 × 105clones . Library clones by sequence determination and bio-informatics preliminary analysis, and with the B lastX and B lastN software homologous comparison, received more than 1,200 of EST sequences, this article will select from more than 20 analysis, Liaoning New strain cashmere goats EST library cDNA library as well as the successful construction of new lines for the study of Liaoning cashmere goats functional genomics and proteomics basis.

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